Method: Logging in Specimens and Record Keeping
June 10, 1990
Rosalie Veile
Purpose:
To keep a written and computerized record of all cell lines,
the dates when cell lines were received and frozen, freezer
locations, and any other important information such as dates
of birth, sex, etc.
Procedure:
- Refer to cell line growth record sheet.
When a cell line arrives or is established from
whole blood, information such as the cell line
number, family position in the pedigree, sex,
date of birth, date arrived, etc., is recorded
on a cell line growth record sheet in a binder
which corresponds with the study group to which
the cell line belongs. Other information is
recorded, such as the dates the cell pellet is
frozen for DNA extraction and for permanent
storage. The freezer locations (in stainless
steel racks) of the cell line aliquots are
recorded to facilitate locating the cell line
at a later date.
- To locate a cell line frozen for DNA extraction,
first look for the location of the cell line on the
master list of all the cell lines in the study group.
This master list is located in the front of the
study group binder to which the cell line belongs.
After the rack location is identified, locate
the position of the cell line in the rack. A
separate binder labelled '-80 Revco' has forms
representing all the racks with in the freezer;
refer to - 80 Revco sheet. When a cell line
is removed it is erased from the -80 rack sheet
and crossed off the master list in the study
group binder.
- A binder to locate frozen cell lines for
permanent storage is labelled '-135 Cryostar'.
The -135 freezer has the capacity to hold 20
racks with 10 boxes in each rack. Each box
has the capacity for 81 cryotubes with one
empty space used for rack orientation. In
the -135 binder, 20 dividers separate the
20 racks, and between each divider are 10
sheets (each labelled -135 Cryostar sheet),
corresponding to the 10 boxes in that rack.
The -135 Cryostar sheet is used to record
the information of what cell lines are in
ach box. On these sheets data such as
kindred number, cell line number, and date
the cell line is frozen is recorded. When
a vial is removed, it is erased off the sheet
and off the master list in the front of
the study group binder.
References:
Veile, R., record keeping, personal notes.
