Human Cell Culture Methods

Logging In Specimens and Record Keeping

Lymphocyte Transformation

Preparation of Lymphoblastoid Cell Lines for Long-Term Storage

Reactivating Frozen Cell Lines and Cell Growth for DNA Preparation

Preparation of Lymphocyte Cell Pellet for Storage

Maintenance of B95-8 Cell Line and Obtaining Virus for Lymphocyte Transformation

Cell Counts Using a Hemocytometer

Removal of Yeast Contamination from Lymphoblast Cultures

Maintaining Lymphoblastoid Cell Lines

Lymphoblastoid Cell Lines from Frozen Whole Blood

Cell Culture Media and Solutions