Lambda Phage Propagation and DNA Preparation Methods

Streaking Lambda Phages

Picking Single Phage Plaques

Liquid Phage Lysates

Gel Assay to Determine DNA Content of Phage Lysates

Titering Lambda Phages

Phage Plate Stock Lysates

Long-Term Lambda Phage Storage

Transfer of Phage DNA from Plaques to Nylon Membrane or Nitrocellulose

Assay for Phage Containing the ß- Galactosidase Gene

Column Method for Lambda Phage DNA Preparation

Growth and Purification of 25-100 µg Lambda Clone DNA

Lambda Methods Media and Solutions

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